Description
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for PSG3 and Horseradish Peroxidase (HRP) conjugated PSG3. The competitive inhibition reaction is launched between with HRP labeled PSG3 and unlabeled PSG3 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PSG3 in the sample. The color development is stopped and the intensity of the color is measured